- ￥2800
- Proteintech
- CL488-68204
- 2025年10月09日
- IF/ICC
- Mouse
- Human, mouse, rat
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human ARF4 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
Human, mouse, rat
- 宿主：
Mouse
- 应用范围：
IF/ICC
- 靶点：
ARF4
- 抗体英文名：
CoraLite® Plus 488-conjugated ARF4 Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated ARF4 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68204 targets ARF4 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag25522 Product name: Recombinant human ARF4 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-180 aa of BC003364 Sequence: MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor 4 |
| 别名 | ADP ribosylation factor 4, ARF2, ARF4 |
| 计算分子量 | 180 aa, 21 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC003364 |
| 基因名称 | ARF4 |
| Gene ID (NCBI) | 378 |
| RRID | AB_3084440 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18085 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.
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