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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human PIK3CD protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 宿主:
Mouse
- 应用范围:
WB, IF/ICC, Indirect ELISA
- 靶点:
PI3 Kinase p110 Delta
- 抗体英文名:
PI3 Kinase p110 Delta Monoclonal antibody, PBS Only
- 抗体名:
PI3 Kinase p110 Delta Monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
67964-1-PBS targets PI3 Kinase p110 Delta in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, Indirect ELISA |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag26995 Product name: Recombinant human PIK3CD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 395-580 aa of BC132919 Sequence: YAVIEKAKKARSTKKKSKKADCPIAWANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPTGTVRSNPNTDSAAALLICLPEVAPHPVYYPALEKILELGRHSECVHVTEEEQLQLREILERRGSGELYEHEKDLVWKLRHEVQEHFPEALARLLLVTKWNKHEDVAQMLYLLCSWPELPVLSAL |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, catalytic, delta polypeptide |
| 别名 | p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta |
| 计算分子量 | 1044 aa, 120 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC132919 |
| 基因名称 | PIK3CD |
| Gene ID (NCBI) | 5293 |
| RRID | AB_2918715 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00329 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验wash). Also, if immunoprecipitating for a kinase reaction, the last wash or two should be in the appropriate kinase buffer. Immunoprecipitation Kinase Assay MAPK 1.) Wash cells 2x with ice-cold PBS. 2.) Add RIPA lysis buffer
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
occurs downstream of growth factor and phosphatidylinositol3 kinase (PI3K) signalling. Activation of the canonicalWnt pathway also inhibits GSK3 activity, although this is notmediated by N-terminal phosphorylation but by protein–proteininteractions (9 –
In-Vitro Adipocytes Differentiation
by activating PI3-kinase and Akt activity. Modulation of the activity of the forkhead transcription factor Foxo1 appears to be necessary for insulin to promote adipocyte differentiation. C/EBPa and PPAR direct the final phase of adipogenesis by activating
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