- ￥980 - 6000
- Proteintech
- 81538-1-RR
- 2025年10月09日
- WB, IHC, IF-P, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, FC (Intra), ELISA
- 靶点：
VDAC1/Porin
- 抗体英文名：
VDAC1/Porin Recombinant monoclonal antibody
- 抗体名：
VDAC1/Porin Recombinant monoclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81538-1-RR targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | voltage-dependent anion channel 1 |
| 别名 | Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | NM_003374 |
| 基因名称 | Porin |
| Gene ID (NCBI) | 7416 |
| RRID | AB_2935560 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P21796 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | J Nutr Biochem Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunctionAuthors - Jingjing Liu |
| rat | WB | Int J Nanomedicine Mitochondria-Rich Microvesicles Alleviate CNI ED by Transferring Mitochondria and Suppressing Local FerroptosisAuthors - Zhenkang Liang |
| rat | WB,IP | Phytother Res Pubescenoside D Ameliorates Myocardial Ischemia-Reperfusion Injury via Preventing the Dissociation of HK2 and Promoting Mitophagy by Targeting GSK-3βAuthors - Juanlan Xiao |
| WB | Plant Physiol The wheat stripe rust effector PstEXLX1 inhibits formate dehydrogenase activity to suppress immunity in wheatAuthors - Jingwei Lu |
B 细胞内抗体产生新机制！两篇 Nature 重磅揭示染色质环挤压导致 V(D)J 重组和抗体类别转换重组
12F3 B 淋巴细胞作为实验对象（该细胞系只能由 IgM 转换成 IgA），敲除 Iα 后用 Anti-CD40-IL-4-TGFβ 作为诱导物，同样利用 GRO-seq，3C-HTGTS 和 ChIP-seq 等测序技术来评估 Iα 转录在 AID 之外的 SαCSR 中的潜在作用。研究人员发现，黏着蛋白介导的环挤压促进 3’RR 激活 IgH 恒定区上游转录单位和 Sm-Sx 配对，并揭示了 CH12F3 细胞只进行 IgA 重组的机制。最后，研究人员又继续探究了 CH12F3NCΔ 细胞
X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏
CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号：MHM24， 2F12； CRIS―3(LFA―lα链，整合素α1) 主要表达细胞：Leu[A5] 分子质量(kDa)和结构：spl80(整合素α) 功 能：与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合，介导细胞黏附；与JAMl结合，参与白细胞穿过内皮细胞
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