- ￥5800
- Proteintech
- 60067-1-PBS
- 2025年10月09日
- WB, IF/ICC, Cytometric bead array, Indirect ELISA
- Mouse
- human, pig, rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NRP1 protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, pig, rabbit
- 宿主：
Mouse
- 应用范围：
WB, IF/ICC, Cytometric bead array, Indirect ELISA
- 靶点：
Neuropilin 1/CD304
- 抗体英文名：
Neuropilin 1/CD304 Monoclonal antibody, PBS Only (Capture)
- 抗体名：
Neuropilin 1/CD304 Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
60067-1-PBS targets Neuropilin 1/CD304 as part of a matched antibody pair:
MP50479-1: 60067-1-PBS capture and 60067-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IF/ICC, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, pig, rabbit |
| 免疫原 |
CatNo: Ag0931 Product name: Recombinant human NRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC007737 Sequence: MERGLPLLCAVLALVLAPAGAFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTN |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | neuropilin 1 |
| 别名 | Neuropilin 1, NRP1, 2H3F6, BDCA4, CD304 |
| 计算分子量 | 103 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC007737 |
| 基因名称 | Neuropilin 1 |
| Gene ID (NCBI) | 8829 |
| RRID | AB_2150840 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14786 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
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文献和实验Immuno-Laser Capture Microdissection
behind the positively stained glands. Magnification x400. Figs. G to I: Microdissection of a single Reed-Sternberg cell stained for CD30 before (G) and after (H) removal. The isolated cell adherent to the membrane (I). Magnification x400 (G & H) and x1000
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
) 0.5 mg/ml human IgG (see recipe ) 1× PBS ( appendix 2E ), 4°C 60% (w/v) sucrose in 1× PBS (store
于细胞阳选，也能用于细胞阴选中。 为了评估 Strep-Tactin®TACS 琼脂糖柱细胞阴选的效率，实验从外周血单个核细胞（PBMC）样品中富集了 CD3- CD56+自然杀伤细胞（NK）。材料为Strep-Tactin®TACS 琼脂糖柱以及生物素化的抗体，仅 10 分钟，便完成了细胞阴选（图 1）。 流程如下： 阴选流程：生物素化抗体与 PBMC 孵育标记非目的细胞，加入至亲和层析柱后，即可收集目的细胞 实验方案 CD3- CD56+ NK 的细胞阴选 · 将 1 x
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