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- 详细信息
- 技术资料
- 免疫原:
Recombinant human LDHA protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
IF/ICC
- 靶点:
LDHA
- 抗体英文名:
CoraLite® Plus 488-conjugated LDHA Polyclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated LDHA Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21799 targets LDHA in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16703 Product name: Recombinant human LDHA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 283-332 aa of BC067223 Sequence: IKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lactate dehydrogenase A |
| 别名 | lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19 |
| 计算分子量 | 332 aa, 37 kDa |
| 观测分子量 | 32-37 kDa |
| GenBank蛋白编号 | BC067223 |
| 基因名称 | LDHA |
| Gene ID (NCBI) | 3939 |
| RRID | AB_3084056 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00338 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).
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