- ￥5800
- Proteintech
- 68395-3-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA, Sample test
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA, Sample test
- 靶点：
Beta-2-Microglobulin
- 抗体英文名：
Beta-2-Microglobulin Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Beta-2-Microglobulin Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
68395-3-PBS targets Beta-2-Microglobulin as part of a matched antibody pair:
MP50108-1: 68395-2-PBS capture and 68395-3-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | beta-2-microglobulin |
| 别名 | B2M, 4D4D5, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2-Microglobulin |
| 计算分子量 | 119 aa, 14 kDa |
| GenBank蛋白编号 | BC032589 |
| 基因名称 | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P61769 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity
Description Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure 1. Carefully remove the growth media from the cell culture. 2. Add an equal volume of PBS and gently mix the PBS solution
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
Beta-Gal staining in primary neurons
Beta-Gal staining in primary neurons Fix cells w/ 4 ml fix soln/10cm or 60 cm dish, 37 C, 5 min Fix soln.: for 100 ml (use w/i 1 wk) 5.4 ml 37 % formaldehyde (Final 2%) 800 ul 25% Glutaraldehyde (fin
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