- ￥5800
- Proteintech
- 66376-1-PBS
- 2025年10月09日
- WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- Mouse
- human, mouse, rat, pig, rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TNNI3 protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Cardiac Troponin I
- 抗体英文名：
Cardiac Troponin I Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Cardiac Troponin I Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
66376-1-PBS targets Cardiac Troponin I as part of a matched antibody pair:
MP51198-1: 66376-2-PBS capture and 66376-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag16758 Product name: Recombinant human TNNI3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | troponin I type 3 (cardiac) |
| 别名 | cTnI, 1D5D6, TNNI3 |
| 计算分子量 | 210 aa, 24 kDa |
| 观测分子量 | 26-28 kDa |
| GenBank蛋白编号 | BC096165 |
| 基因名称 | Cardiac Troponin I |
| Gene ID (NCBI) | 7137 |
| RRID | AB_2881753 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P19429 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.
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文献和实验Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
Primary antibodies against: Cardiac troponin T (cTnT, mouse monoclonal, NeoMarkers, Ms‐295, 0.2 mg/ml, 1:250
50μL。 封闭液：用PBS将试剂盒中的封闭存储液（Blocking stock solution R）稀释10倍，每孔200μL。 抗体稀释液：注意，只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液（Dilution buffer R）稀释10倍 检测抗体（Biotinylated detector antibody，Secondary antibody）：照U-Cytech说明书，加入双蒸水溶解。使用时，用抗体稀释液稀释100倍，每孔100μL。 酶联亲和
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