- ￥2800
- Proteintech
- CL488-21774
- 2025年10月09日
- IF-P, FC (Intra)
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human L-VOCC protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
IF-P, FC (Intra)
- 靶点：
L-VOCC
- 抗体英文名：
CoraLite® Plus 488-conjugated L-VOCC Polyclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated L-VOCC Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21774 targets L-VOCC in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16455 Product name: Recombinant human L-VOCC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1835-2135 aa of BC146846 Sequence: EVKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium channel, voltage-dependent, L type, alpha 1C subunit |
| 别名 | CACNA1C, CCHL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CACNL1A1, CACN2 |
| 计算分子量 | 249 kDa |
| GenBank蛋白编号 | BC146846 |
| 基因名称 | CACNA1C |
| Gene ID (NCBI) | 775 |
| RRID | AB_3084055 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13936 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验on Veeco DI SPMLab software. 5. Photographs The photographs, showing difference in intensity of Alexa488 signal on glass, SIF, and SACS, were taken with Canon 300D® digital SLR camera. The illumination source was Ti: Sapphire laser (Mai Tai
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
from various vendors,including BD Transduction Laboratories, FranklinLakes, NJ; R&D Systems; and Santa Cruz Biotechnology,Santa Cruz, CA). 11. Appropriate conjugated secondary antibody for detection. 3. Methods 3.1. Production of Wnt3A CM 1. Grow L-
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