- ￥2800
- Proteintech
- CL594-66632
- 2025年10月09日
- FC (Intra)
- Mouse
- human, mouse, rat
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human NEK2 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
FC (Intra)
- 靶点：
NEK2
- 抗体英文名：
CoraLite®594-conjugated NEK2 Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated NEK2 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66632 targets NEK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag21476 Product name: Recombinant human NEK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-445 aa of BC043502 Sequence: MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NIMA (never in mitosis gene a)-related kinase 2 |
| 别名 | Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21, EC:2.7.11.1 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC043502 |
| 基因名称 | NEK2 |
| Gene ID (NCBI) | 4751 |
| RRID | AB_2923922 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P51955 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEK2, also named as HSPK 21, belongs to the NEK family. NEK2 is well recognized as a multifunctional protein with roles in cell cycle regulation, such as centrosome duplication and separation, microtubule stabilization, kinetochore attachment and spindle assembly checkpoint. NEK2 is highly expressed in several cancer types, such as cholangiocarcinoma, breast, colorectal, and pancreatic cancer. NEK2 in mammals has three splice variants: NEK2A, NEK2B, and NEK2C. NEK2A is evenly distributed within the nuclei and cytoplasm, while NEK2B is mainly distributed in the cytoplasm, and NEK2C is mainly distributed in the nuclear region. As NEK2A, NEK2B and NEK2C exhibit overlapping or identical substrate usage, these variants are collectively referred to here as NEK2 (PMID:31092416).
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