- ￥3200
- Proteintech
- CL488-24905
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human, mouse
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human GRAMD1B protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
GRAMD1B
- 抗体英文名：
CoraLite® Plus 488-conjugated GRAMD1B Polyclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated GRAMD1B Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-24905 targets GRAMD1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20670 Product name: Recombinant human GRAMD1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 235-504 aa of BC107480 Sequence: YVPPDDDFNTMGYCEEIPVEENEVNDSSSKSSIETKPDASPQLPKKSITNSTLTSTGSSEAPVSFDGLPLEEEALEGDGSLEKELAIDNIMGEKIEMIAPVNSPSLDFNDNEDIPTELSDSSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFQRDFMEQRRFSDIIFHPWKKEENGNQSRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKSRLRVSTELRY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GRAM domain containing 1B |
| 别名 | UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B |
| 计算分子量 | 738 aa, 85 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC107480 |
| 基因名称 | GRAMD1B |
| Gene ID (NCBI) | 57476 |
| RRID | AB_3672780 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3KR37 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.
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