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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2c
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
Ly-6C
- 抗体英文名:
CoraLite® Plus 488 Anti-Mouse Ly-6C (HK1.4)
- 抗体名:
CoraLite® Plus 488 Anti-Mouse Ly-6C (HK1.4)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥920.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2310.0 |
经过测试的应用
| Positive FC detected in | mouse bone marrow cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65296 targets Ly-6C in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2c |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte antigen 6 complex, locus C1 |
| 别名 | AA682074, AA959465, Ly 6C, Ly6c, Ly6c1 |
| GenBank蛋白编号 | BC010764 |
| 基因名称 | Ly-6C |
| Gene ID (NCBI) | 17067 |
| RRID | AB_3672875 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P0CW02 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).
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文献和实验,于室温处理 1 小时进行终止。+4°C 环境下,将神经元细胞与神经元标志微管相关蛋白 2 (microtubule-associated protein 2,MAP-2) (英国剑桥Abcam,ab11267, HM-2,1:600 稀释)小鼠单克隆抗体共同孵育,孵育过夜。第 2 天,将细胞与山羊抗小鼠二抗(Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Invitrogen,1:250 稀释)共同孵育。使用DMI6000 B 倒置显微镜(德国,Leica
3dCsv0oa0K/SASPFgw5eAjyccPfMLqyavxf7R49BqQoH0J3/fyZ517iDxBPgb4fEs+OWkQn4OmDv9lfQKlcXOe2vw+rIO3QcY0qnzIorgZ9OYfXeX8j9+z6+1puOKQ1yh34QK24KSR6patWqUh9Xr15Ncl+p1YGvry8JBUpQJ6FBPLS1Q6OSJlST4+NDX6zPnuPly5d488YJri6uePv2Le7euYN1q0Ojj6mQFiVXrlwoWKggSpQsiRo1aqBR48
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