- ￥2800
- Proteintech
- CL488-67999
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- human, mouse, rat, pig
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human RPA2 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
RPA2
- 抗体英文名：
CoraLite® Plus 488-conjugated RPA2 Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated RPA2 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67999 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag24783 Product name: Recombinant human RPA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 80-270 aa of BC001630 Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | replication protein A2, 32kDa |
| 别名 | 1B9H11, REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC001630 |
| 基因名称 | RPA2 |
| Gene ID (NCBI) | 6118 |
| RRID | AB_2923854 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15927 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.
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