- ￥1200
- Proteintech
- CL647-66002
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- recombinant protein
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant aequorea victoria GFP tag protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
recombinant protein
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
GFP tag
- 抗体英文名：
CoraLite® Plus 647-conjugated GFP tag Monoclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated GFP tag Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66002 targets GFP tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | recombinant protein |
| 免疫原 |
CatNo: Ag2128 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Domain: of Sequence: |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GFP tag |
| 别名 | eGFP, GFP, CFP, eYFP, GFP-tag |
| 计算分子量 | 26 kDa |
| GenBank蛋白编号 | M62653 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3673639 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42212 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 647.
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