- ￥2800
- Proteintech
- CL594-67996
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- Human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MYBBP1A protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
Human, mouse, rat
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
MYBBP1A
- 抗体英文名：
CoraLite®594-conjugated MYBBP1A Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated MYBBP1A Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67996 targets MYBBP1A in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag6008 Product name: Recombinant human MYBBP1A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 979-1328 aa of BC050546 Sequence: STALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRRQACLLLQKTLSMREVRSCFEDPEWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQQGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGTPAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQVNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | MYB binding protein (P160) 1a |
| 别名 | PAP2, Myb-binding protein 1A, Myb binding protein 1A, 1H2D10 |
| 计算分子量 | 149 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC050546 |
| 基因名称 | MYBBP1A |
| Gene ID (NCBI) | 10514 |
| RRID | AB_2923939 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BQG0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].
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