- ￥1500 - 3280
- Proteintech
- 81219-2-RR
- 2025年10月09日
- WB, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
Phospho-SHP2 (Tyr580)
- 抗体英文名：
Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody
- 抗体名：
Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | PDGF treated NIH/3T3 cells, PDGF treated C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81219-2-RR targets Phospho-SHP2 (Tyr580) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, non-receptor type 11 |
| 别名 | P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580) |
| 计算分子量 | 597 aa, 68 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC008692 |
| 基因名称 | PTPN11 |
| Gene ID (NCBI) | 5781 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q06124 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
30s 延伸 72℃ 30s 结束延伸 72℃ 10min 1 保存 4℃ 1 如果筛选的是Mut+，将会出现两条带，一个是目的基因，另一个是2.2kb的AOX1基因，空质粒会出现以下条带（pPICZαA 588 bp)，将两者的大小加在一起来解释出现的实验结果。 我的结果是空载体的有大约580多的条带，但重组子的PCR只有将近1000bp的条带（我的目的基因是400bp），没有2.2kb的条带。
, Young, and Davis (1985) DNA Cloning, Vol. 1, 56-110.DH1- F-, recA1, endA1, gyrA96, thi-1, hsdR17 (rk-, mk+), supE44, relA1, l--for plasmid transformation, grows well on L broth and plates.-Hanahan (1983) J. Mol. Biol. 166, 557-580.XL1Blue-MRF' - D(mcrA
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