- ￥5800
- Proteintech
- 66445-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Indirect ELISA
- Mouse
- human, mouse, rat, pig, canine
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human JUP protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, canine
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, Indirect ELISA
- 靶点：
Gamma Catenin
- 抗体英文名：
Gamma Catenin Monoclonal antibody, PBS Only
- 抗体名：
Gamma Catenin Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
66445-1-PBS targets Gamma Catenin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, Indirect ELISA |
| 经测试反应性 | human, mouse, rat, pig, canine |
| 免疫原 |
CatNo: Ag1627 Product name: Recombinant human JUP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-745 aa of BC011865 Sequence: LVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | junction plakoglobin |
| 别名 | JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma |
| 计算分子量 | 82 kDa |
| 观测分子量 | 82 kDa |
| GenBank蛋白编号 | BC011865 |
| 基因名称 | Gamma Catenin |
| Gene ID (NCBI) | 3728 |
| RRID | AB_2881814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P14923 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Irradiating Cells - Once cells are confluent tighten the lid of the flasks and take up to the gamma source in the animal house on the third floor (PMCI), if you have a number of flasks you can trypsinise all the flasks and place
containing 10ml of PMEF media. Incubate at 37oC for two days or until confluent IRRADIATING CELLS Once cells are confluent tighten the lid of the flask and take up to the gamma source in the animal house on the third floor (PMCI) Irradiate
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
技术资料暂无技术资料 索取技术资料
