- ￥1500 - 3280
- Proteintech
- 80706-2-RR
- 2025年10月09日
- WB, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
Phospho-c-SRC (Tyr530)
- 抗体英文名：
Phospho-c-SRC (Tyr530) Recombinant monoclonal antibody
- 抗体名：
Phospho-c-SRC (Tyr530) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | LPS treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80706-2-RR targets Phospho-c-SRC (Tyr530) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| 别名 | c-SRC (Tyr530), p c-SRC (Tyr530), p-c-SRC (Tyr530), Phospho c-SRC (Tyr530), Phospho-SRC (Tyr530) |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC011566 |
| 基因名称 | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| RRID | AB_3670485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P12931 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Src is a 60 kDa protein encoded by the Src gene belonging to a family of non-RTK known as SFK. As a typical protein, the polypeptide sequence of c-Src comprises an N-terminal head and a C-terminal tail but has four Src homology (SH) domains numbered 1, 2, 3, and 4. The C-terminal tail consists of a carboxy group that is short and connected to the SH1 domain. Additionally, the C-terminal domain serves as an anchor for a phosphotyrosine moiety, specifically Tyr530 in human Src (or Tyr527 in chicken). (PMID: 38201459)
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,human | WB | J Ethnopharmacol Paeoniflorin inhibits chronic restraint stress-induced progression of hepatocellular carcinoma through suppressing norepinephrine-induced activation of hepatic stellate cells via SRC/AKT/ERK pathwaysAuthors - Yujun Luo |
| mouse | WB | Neurol Res Exploring neuroprotective effects of PP2 in ischemic stroke via bioinformatics and experimental validationAuthors - Shiyan Zhao |
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
细胞中c-abl、ltk、c-kit和其它原癌基因蛋白产物。这些PTKs的确切功能还不清楚。 表8-2 同src家族PTKs相连接的细胞表面蛋白 细胞表面蛋白 细胞类型 src家族PTK TCR-CD3 T Fyn CD2 T Fyn CD4 T Lck CD8 T Lck CD45 T Fyn、Lck BCR B Blk、Lyn(Fyn)、Lck FcγRⅢ（CD16） NK Fyn IL-2R T，NK Lck、Lyn FcεR I 肥大细胞、嗜碱性粒细胞 Yes、Lyn
因为短的肽标签对目标蛋白的结构影响最小，而大的亲和标签可能限制重组蛋白的折叠，影响蛋白质的生物学功能。（2）亲和标签对蛋白质稳定性的影响，亲和标签的加入是否会影响到目标蛋白的正确折叠，是促进目标蛋白的可溶性，还是容易形成包涵体，会不会造成氨基酸C和N端破坏，使目的蛋白表达不完全。（3）亲和标签所融合的位置，亲和标签可以加在N端，可以加在C端，也进行串联。多数情况下蛋白质的N端区域对蛋白质的功能不是太重要，所以在N端进行融合标签的融合常常能保留蛋白质的生物学功能。由于N端DNA序列对蛋白质的转录
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