- ￥5800
- Proteintech
- 80258-2-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
TNF-alpha
- 抗体英文名：
Mouse TNF-alpha Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Mouse TNF-alpha Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
80258-2-PBS targets TNF-alpha as part of a matched antibody pair:
MP00682-1: 80258-3-PBS capture and 80258-2-PBS detection (validated in Cytometric bead array)
MP00682-2: 80258-4-PBS capture and 80258-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg31366 Product name: Recombinant Mouse TNF-alpha protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 80-235 aa of NM-013693 Sequence: LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor |
| 别名 | Tnf, Tnfa, DIF, TNF a, TNF alpha |
| GenBank蛋白编号 | NM-013693 |
| 基因名称 | TNF-alpha |
| Gene ID (NCBI) | 21926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06804 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验1. 抗原及酶标记抗体 ①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No.A0423); ②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No.P0260);均为 DAKO 公司产品，使用时按照说明书要求稀释。 4. 洗涤液(含0.05%Tween 20的PBS)：1LPBS 中加入Tween-20 500μL。 5. 封闭液(含1%牛血
蛋白质，加酶标抗抗体，保温后洗涤，加底物保温30分钟后，加酸或碱终止酶促反应，用目测或光电比色测定抗体含量。三、仪器、原料和试剂仪器聚苯乙烯96孔酶标板、微量移液管、酶联免疫阅读仪、水浴锅。原料单克隆抗体试剂1、 抗原及酶标记抗体①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No．A0423)；②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No．P0260)；均为DAKO 公司产品，使用时按照说明书要求稀释。4、 洗涤
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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