- ￥3200
- Proteintech
- CL488-15508
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Transgelin-2-specific
- 抗体英文名：
CoraLite® Plus 488-conjugated Transgelin-2-specific Polyclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Transgelin-2-specific Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-15508 targets Transgelin-2-specific in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transgelin 2 |
| 别名 | Transgelin 2, TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | NM_003564 |
| 基因名称 | Transgelin-2/TAGLN2 |
| Gene ID (NCBI) | 8407 |
| RRID | AB_3083971 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P37802 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
conjugated species‐specific secondary antibodies (Invitrogen or Li‐COR, http://www.licor.com) NeutrAvidin, DyLight 800 conjugate (Thermo Scientific
on Veeco DI SPMLab software. 5. Photographs The photographs, showing difference in intensity of Alexa488 signal on glass, SIF, and SACS, were taken with Canon 300D® digital SLR camera. The illumination source was Ti: Sapphire laser (Mai Tai
表达系统（178-541aa）：CSB-EP745804 ENL1 多克隆抗体货号（兔多抗）： No conjugated：CSB-PA745804LA01ENL HRP conjugated：CSB-PA745804LB01ENL FITC conjugated：CSB-PA745804LC01ENL Biotin conjugated：CSB-PA745804LD01ENL 参考文献： [1] Systems Genomics
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