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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
CX3CL1
- 抗体英文名:
CX3CL1 Recombinant monoclonal antibody
- 抗体名:
CX3CL1 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse lung tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83738-5-RR targets CX3CL1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X3-C motif) ligand 1 |
| 别名 | Scyd1, Cxc3, CX3C, ABCD 3, 240738E2 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 55-75 kDa |
| GenBank蛋白编号 | NM_009142.3 |
| 基因名称 | Cx3cl1 |
| Gene ID (NCBI) | 20312 |
| RRID | AB_3671337 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O35188 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).
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文献和实验ml)lml,再从鸡的翼下静脉取血0.5--1ml,取出后放入刻度离心管中,再加入2--3ml Alsver液,使总量为4--5ml,混匀并封口,置于4℃冰箱内可供一周内使用,实验时取贮备的鸡血细胞lml,加入4ml85%生理盐水,混匀后以1200rr/min离心5min,去上清液后,最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次,最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞,可先用0.9%
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