- ￥750 - 1500
- Proteintech
- Biotin-FcA65569
- 2025年10月09日
- FC
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Biotin
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CD3
- 抗体英文名：
FcZero-rAb™ Biotin Anti-Human CD3 (OKT3) Rabbit IgG Recombinant Antibody
- 抗体名：
FcZero-rAb™ Biotin Anti-Human CD3 (OKT3) Rabbit IgG Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥750.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1500.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-FcA65569 targets CD3 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD3e molecule, epsilon (CD3-TCR complex) |
| 别名 | CD3E, CD3 epsilon, CD3-epsilon, OKT3, T3E |
| 计算分子量 | 207 aa, 23 kDa |
| GenBank蛋白编号 | BC049847 |
| 基因名称 | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| 偶联类型 | Biotin |
| 最大激发/发射波长 | - |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07766 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
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The Following Protocol can be used to couple either FITC or Biotin to IgG. The same method is used for both, and when discrepancies occur, they will be noted. 1. Dissolve 2mg of IgG in 1mL of PBS (pH
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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