- ￥1200
- Proteintech
- HRP-66006
- 2025年10月09日
- WB
- Mouse
- recombinant protein
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
recombinant protein
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
HA Tag
- 抗体英文名：
HRP-conjugated HA Tag Monoclonal antibody
- 抗体名：
HRP-conjugated HA Tag Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66006 targets HA Tag in WB applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB |
| 文献引用应用 | WB |
| 经测试反应性 | recombinant protein |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | HA Tag |
| 别名 | YPYDVPDYA |
| 基因名称 | HA Tag |
| Gene ID (NCBI) | 6 |
| RRID | AB_3086576 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | HATAG |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
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文献和实验| Species | Application | Title |
|---|---|---|
| WB | J Cancer KRT80 Promotes Lung Adenocarcinoma Progression and Serves as a Substrate for VCPAuthors - Shanhua Huang | |
| WB | Cell Death Dis GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinomaAuthors - Jiaru Hu |
凝集素，四聚体球蛋白，含有糖基结合位点，因此 ConA 是一种糖类结合蛋白。ConA 磁珠能与细胞膜上的糖蛋白结合，从而吸附细胞，使对细胞的实验操作可视化，减少在后续实验过程中的细胞的损失。 不建议使用离心法代替 ConA beads，因为离心法不适合于低细胞数，在洗涤的过程中，损失很大，而且大肠杆菌残留的 DNA 无法进行可靠的校准。 5.做 ChIP-seq 的时候不需要二抗，CUT&Tag 二抗的作用是什么？二抗是必须的吗？可以用带修饰（比如 HRP 修饰）的二抗
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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