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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
FITC Plus Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD11a
- 抗体英文名:
FITC Plus Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody
- 抗体名:
FITC Plus Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥800.0 |
经过测试的应用
| Positive FC detected in | human peripheral blood leukocyte |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65563 targets CD11a in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| 别名 | Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L |
| 计算分子量 | 129 kDa |
| GenBank蛋白编号 | BC008777 |
| 基因名称 | CD11a |
| Gene ID (NCBI) | 3683 |
| ENSEMBL Gene ID | ENSG00000005844 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20701 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
A Protein G Human IgG1 ++++ ++++ Human IgG2 ++++ ++++ Human IgG3 - ++++ Human IgG4 ++++ ++++ Mouse IgG1 + ++++ Mouse IgG2a
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