- ￥1350 - 3000
- Proteintech
- 28596-1-AP
- 2025年10月09日
- WB, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TMEM200C protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
TMEM200C
- 抗体英文名：
TMEM200C Polyclonal antibody
- 抗体名：
TMEM200C Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28596-1-AP targets TMEM200C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag29577 Product name: Recombinant human TMEM200C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 385-621 aa of NM_001080209 Sequence: QGGRDRGGDAEGASCSWQRPPGERGSQEIPRGELDLSMTNLRGAEGSMRGARREPEEPEGAVAARAARGQGGRLPRTGRYAALRRRSTSGLPDYRAPPSPEPPPSPGSADPDSSPLAKAASPSPPLRLEGSPPTRRDSGSSQSDDPSSSNKGYTPLREAGTSTESVLDAVAGQTRDSAVAAPVLGAEQSSPEGASQEPPTAEQPQPVQRQFTNKEKLIMISRSHAIGVEEELESTGI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | two transmembrane domain family member A |
| 别名 | TTMA, Transmembrane protein TTMA, Transmembrane protein 200C |
| 计算分子量 | 64 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_001080209 |
| 基因名称 | TTMA |
| Gene ID (NCBI) | 645369 |
| RRID | AB_3669664 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NKL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM200C (Transmembrane protein 200C) may be an integral component of the membrane.
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文献和实验AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
B.ELISA结果 C.1,000~1,200ml抗血清 SC1051 Protein A亲和纯化多克隆抗体制备 服务内容 交付 a.多肽合成（少于15个氨基酸，纯度大于85%，10mg） b.C末段或N末段半胱氨酸偶联KLH
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
% (w/v) maltose + 10 mM MgSO4 with a single colony. Grow at 37°C, shaking for 4-6 hours (do not grow beyond an OD600 of 1.0). Alternatively, grow overnight at 30°C, shaking at 200 rpm. Phage can adhere to dead cells as well as to live ones
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