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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human tubulin-Alpha protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, zebrafish, rat
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
Alpha Tubulin
- 抗体英文名:
Alpha Tubulin Polyclonal antibody
- 抗体名:
Alpha Tubulin Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues |
| Positive IHC detected in | rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | human, mouse, zebrafish, rat |
| 免疫原 |
CatNo: Ag8695 Product name: Recombinant human tubulin-Alpha protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-449 aa of BC004949 Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubulin, alpha 1c |
| 别名 | alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c |
| 计算分子量 | 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC004949 |
| 基因名称 | Alpha Tubulin |
| Gene ID (NCBI) | 84790 |
| RRID | AB_2303957 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQE3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
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