- ￥3200
- Proteintech
- CL594-14577
- 2025年10月09日
- IF-P
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SF3B3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
IF-P
- 靶点：
SF3B3
- 抗体英文名：
CoraLite®594-conjugated SF3B3 Polyclonal antibody
- 抗体名：
CoraLite®594-conjugated SF3B3 Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-14577 targets SF3B3 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5980 Product name: Recombinant human SF3B3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-399 aa of BC003146 Sequence: EDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor 3b, subunit 3, 130kDa |
| 别名 | KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130 |
| 计算分子量 | 136 kDa |
| 观测分子量 | 130-135 kDa |
| GenBank蛋白编号 | BC003146 |
| 基因名称 | SF3B3 |
| Gene ID (NCBI) | 23450 |
| RRID | AB_3084641 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15393 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.
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