- ￥1020 - 2540
- Proteintech
- AB-65119
- 2025年10月09日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Atlantic Blue™ Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
PD-1/CD279
- 抗体英文名：
Atlantic Blue™ Anti-Human PD-1/CD279 (J110)
- 抗体名：
Atlantic Blue™ Anti-Human PD-1/CD279 (J110)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥1020.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2540.0 |
经过测试的应用
| Positive FC detected in | PHA treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
AB-65119 targets PD-1/CD279 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | programmed cell death 1 |
| 别名 | CD279, PD1, PD-1, hPD 1, hPD l |
| 计算分子量 | 288 aa, 32 kDa |
| GenBank蛋白编号 | BC074740 |
| 基因名称 | PD-1 |
| Gene ID (NCBI) | 5133 |
| RRID | AB_3672340 |
| 偶联类型 | Atlantic Blue™ Fluorescent Dye |
| 最大激发/发射波长 | 404 nm / 458 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q15116 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
cut paraffin sections and deparaffinize with xylene and graded EtOH into PBS. Block slides with 1/20x serum/PBS (use the appropriate blocking serum, i.e. same as 1º Ab), 10'. Wash in PBS 2' x3. Add digoxigenin labeled 1º antiobody.
Protocol for anti-HA antibody Western Blotting
SJF 4/00 1) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of 1litre) at 150 volts until leading bromophenol blue band is nearing the bottom edge of the glass plates or according to the expected migration of the protein
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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