- ￥2800
- Proteintech
- CL594-66292
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- human
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human BDNF protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
BDNF
- 抗体英文名：
CoraLite®594-conjugated BDNF Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated BDNF Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | PC-12 cells |
| Positive FC (Intra) detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66292 targets BDNF in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11329 Product name: Recombinant human BDNF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 21-247 aa of BC029795 Sequence: MKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHMIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | brain-derived neurotrophic factor |
| 别名 | Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5 |
| 计算分子量 | 247 aa, 28 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC029795 |
| 基因名称 | BDNF |
| Gene ID (NCBI) | 627 |
| RRID | AB_2934722 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P23560 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
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