- ￥920 - 2310
- Proteintech
- AB-65098
- 2025年10月09日
- FC
- Rat
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Atlantic Blue™ Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
LAG-3/CD223
- 抗体英文名：
Atlantic Blue™ Anti-Mouse LAG-3/CD223 (C9B7W)
- 抗体名：
Atlantic Blue™ Anti-Mouse LAG-3/CD223 (C9B7W)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥920.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2310.0 |
经过测试的应用
| Positive FC detected in | plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
AB-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte-activation gene 3 |
| 别名 | CD223, LAG-3, Ly66, Lag3, LAG 3 |
| GenBank蛋白编号 | BC120591 |
| 基因名称 | CD223 |
| Gene ID (NCBI) | 16768 |
| RRID | AB_2935166 |
| 偶联类型 | Atlantic Blue™ Fluorescent Dye |
| 最大激发/发射波长 | 404 nm / 458 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q61790 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Ususally 1:1000 (v/v) in PBS. Wash in PBS 2' x3. Equilibrate slides in Buffer 1, RT x 5'. Incubate slides in Blocking Buffer, RT x 30' Incubate with anti-Dig-AP conjugate (1:2000 in Blocking buffer.), 2 hrs. RT, or o.n. at 4ºC.
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
Performing Bronchoalveolar Lavage in the Mouse
A, methanol 100%) Reagent HMS‐B (Eosin 0.075%, H 2 Od) Reagent HMS‐C (methylene blue 0.06%, toluidine blue 0.04%, H 2 Od
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