FcZero-rAb™ CoraLite® Plus 488
Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody CL488-FcA65612- ￥480 - 960
- Proteintech
- CL488-FcA65612
- 2025年10月09日
- FC
- Rabbit
- human, non-human primates
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, non-human primates
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CD16
- 抗体英文名：
FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody
- 抗体名：
FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥960.0 |
经过测试的应用
| Positive FC detected in | human peripheral blood leukocyte |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-FcA65612 targets CD16 in FC applications and shows reactivity with human, non-human primates samples.
| 经测试应用 | FC |
| 经测试反应性 | human, non-human primates |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| 别名 | 3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII |
| 计算分子量 | 254 aa, 29 kDa |
| GenBank蛋白编号 | BC017865 |
| 基因名称 | CD16a |
| Gene ID (NCBI) | 2214 |
| ENSEMBL Gene ID | ENSG00000203747 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08637 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).
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文献和实验was measured in front-face configuration. The measured fluorescence signal was a result of a specific interaction. To verify this, we performed the immunoassay using the same labeled antibodies, but with a nonspecific antigen, rabbit IgG
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂：PBS(磷酸盐缓冲液pH7.4)（实验室常规配置）；非免疫山羊血清；Triton X-100（sigma）；BSA抗体稀释液（实验室常规配置）；A蛋白抗体（Santa cruz）；B蛋白抗体（Abcam）；Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen)；Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
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