- ￥3200
- Proteintech
- Biotin-55179
- 2025年10月09日
- IHC
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Biotin Plus
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
IHC
- 靶点：
ATP1A2-Specific
- 抗体英文名：
Biotin Plus-conjugated ATP1A2-Specific Polyclonal antibody
- 抗体名：
Biotin Plus-conjugated ATP1A2-Specific Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-55179 targets ATP1A2-Specific in IHC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| 别名 | ATP1A2, Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2 |
| 计算分子量 | 112 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | NM_000702 |
| 基因名称 | ATP1A2 |
| Gene ID (NCBI) | 477 |
| 偶联类型 | Biotin Plus |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity Purified |
| UNIPROT ID | P50993 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.
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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
Conjugation of Antibodies to Biotin
Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
for each species of primary antibody used. The biotin molecule is small and can be easily conjugated to immunoglobulin by amino substitution at alkaline pH without the loss of immunoglobulin activity. These secondary antibodies are quite inexpensive, readily
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