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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR
- 靶点:
Di-Methyl-Histone H3 (Arg2)
- 抗体英文名:
Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody, PBS Only
- 抗体名:
Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
84702-1-PBS targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3 |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Reagents 100-bp DNA size ladder (Promega) Agarose Antisera (affinity-purified) These should be raised against histone peptides with mono-, di-, or trimethylation or acetylation at a specific lysine/arginine residue
解开双链DNA,产生单/低聚核小体片段,而核小体本身由于DNA与组蛋白H1、H2A、H2B、H3和H4形成紧密复合物而不被核酸内切酶裂解。采用双抗体夹心酶免疫法,应用小鼠抗DNA和抗组蛋白的单克隆抗体,与核小体片段形成夹心结构,可特异性检测细胞溶解物中的单/低聚核小体,从而判断细胞是否发生凋亡。 【材料与试剂】 (1) 生物素标记的小鼠抗-组蛋白单克隆抗体; (2) 过氧化物酶(-POD)标记的小鼠抗DNA单克隆抗体; (3) 链霉亲合素包被的微孔板; (4) DNA-组蛋白复合
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