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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, Indirect ELISA
- 靶点:
CD3
- 抗体英文名:
CD3 Recombinant monoclonal antibody, PBS Only
- 抗体名:
CD3 Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
84505-4-PBS targets CD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD3e molecule, epsilon (CD3-TCR complex) |
| 别名 | CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon |
| 计算分子量 | 207 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC049847 |
| 基因名称 | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P07766 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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