- ￥3600 - 5000
- Proteintech
- 65578-1-PBS
- 2025年10月09日
- FC
- Mouse
- rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
rat
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD45RA
- 抗体英文名：
Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody, PBS Only
- 抗体名：
Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody, PBS Only
- 规格：
500ug/1mg
| 规格： | 500ug | 产品价格： | ￥3600.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥5000.0 |
产品信息
65578-1-PBS targets CD45RA in FC applications and shows reactivity with rat samples.
| 经测试应用 | FC |
| 经测试反应性 | rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | CD45, Lca, Ptprc, L CA, OX-33 |
| 计算分子量 | 143 kDa |
| GenBank蛋白编号 | XM_006249910 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 24699 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04157 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
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