- ￥1500 - 3280
- Proteintech
- 83734-2-RR
- 2025年10月09日
- WB, IHC, FC (Intra), ELISA
- Rabbit
- human
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human USP4 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), ELISA
- 靶点：
USP4
- 抗体英文名：
USP4 Recombinant monoclonal antibody
- 抗体名：
USP4 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | THP-1 cells, NCI-H1299 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | U-251 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83734-2-RR targets USP4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28297 Product name: Recombinant human USP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 567-779 aa of BC125131 Sequence: VCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 4 (proto-oncogene) |
| 别名 | Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12 |
| 计算分子量 | 963 aa, 109 kDa |
| 观测分子量 | 109 kDa |
| GenBank蛋白编号 | BC125131 |
| 基因名称 | USP4 |
| Gene ID (NCBI) | 7375 |
| RRID | AB_3671334 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13107 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP4, also known as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).
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