- ￥2800
- Proteintech
- CL532-82975
- 2025年10月09日
- IF/ICC, IF-P
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ARG1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite®532 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
IF/ICC, IF-P
- 靶点：
Arginase-1
- 抗体英文名：
CoraLite®532-conjugated Arginase-1 Recombinant monoclonal antibody
- 抗体名：
CoraLite®532-conjugated Arginase-1 Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL532-82975 targets Arginase-1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, IF-P |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8595 Product name: Recombinant human ARG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC005321 Sequence: MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | arginase, liver |
| 别名 | ARG1, Arginase 1, liver Arginase, 4M7, ARG 1 |
| 计算分子量 | 236aa,25 kDa; 322aa,35 kDa |
| 观测分子量 | 35-36 kDa |
| GenBank蛋白编号 | BC005321 |
| 基因名称 | Arginase-1 |
| Gene ID (NCBI) | 383 |
| RRID | AB_3673400 |
| 偶联类型 | CoraLite®532 Fluorescent Dye |
| 最大激发/发射波长 | 537 nm / 560 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05089 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).
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文献和实验2. 以标准品4000 、 2000 、 1000 、 500 、 250 、 125 、 62.5 、 0 pg /ml 为横坐标， OD 值为纵坐标，在坐标纸上作图，画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 Arginase 含量 ，再乘上稀释倍数即可 。 试剂盒性能 1. 灵敏度 ： 最小的Arginase 检测浓度小于 31p g/ml。 2. 特异性： 可同时检测重组或天然的人 Arginase
的强大之处在于完整的细胞器，可利用活性线粒体提供持续的能量供应。此外，微粒体（内质网重组形成的囊泡）还能够对复杂蛋白质进行折叠和翻译后修饰，包括二硫键和糖基化。 ALiCE® kit components: • Two expression vectors, pALiCE01 for expression in cytosolic fraction and pALiCE02 that offers the opportunity to express protein with post
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