- ￥5800
- Proteintech
- 83706-6-PBS
- 2025年10月09日
- WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Beta galactosidase protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Beta galactosidase
- 抗体英文名：
Beta galactosidase Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Beta galactosidase Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
83706-6-PBS targets Beta galactosidase as part of a matched antibody pair:
MP00647-4: 83706-4-PBS capture and 83706-6-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag7792 Product name: Recombinant human Beta galactosidase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 329-677 aa of BC007493 Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | galactosidase, beta 1 |
| 别名 | GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP |
| 计算分子量 | 76 kDa |
| 观测分子量 | 62-67 kDa |
| GenBank蛋白编号 | BC007493 |
| 基因名称 | GLB1/Beta-galactosidase |
| Gene ID (NCBI) | 2720 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P16278 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.
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文献和实验Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity
Description Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure 1. Carefully remove the growth media from the cell culture. 2. Add an equal volume of PBS and gently mix the PBS solution
Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide") Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity. 1. Wash cells 2 - 3 x with PBS (2 ml
上带有Ampr 和lacZ基因,故重组子的筛选采用Amp抗性筛选与α-互补现象筛选相结合的方法。 因pBS带有Ampr 基因而外源片段上不带该基因,故转化受体菌后只有带有pBS DNA的转化子才能在含有Amp的LB平板上存活下来;而只带有自身环化的外源片段的转化子则不能存活。此为初步的抗性筛选。 pBS上带有β-半乳糖苷酶基因(lacZ)的调控序列和β-半乳糖苷酶N端146个氨基酸的编码序列。这个编码区中插入了一个多克隆位点,但并没有破坏
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