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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
CD22
- 抗体英文名:
CoraLite® Plus 750-conjugated CD22 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 750-conjugated CD22 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83303 targets CD22 in WB applications and shows reactivity with human samples.
| 经测试应用 | WB |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD22 molecule |
| 别名 | CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22 |
| 计算分子量 | 95kd |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | NM_001771.4 |
| 基因名称 | CD22 |
| Gene ID (NCBI) | 933 |
| RRID | AB_3673781 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20273 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
dH2 O) Denaturing solution (0.5 N NaOH, 1.5 M NaCl) (dissolve 43.83 g NaCl in 300 ml dH2 O, add 50 ml 10 N NaOH stock solution , dH2 O ad 500 ml) Neutralizing solution (1.5 M NaCl, 0.5 M Tris ・ Cl [pH 7.4]) (100 ml dH
体内后,通过糖苷键结合参与代谢,转换为葡萄糖醛酸轭合物或硫酸轭合物。所以β-受体激动剂的检测通常需要酶解步骤,β-葡萄糖醛酸酶是一种可以使葡萄糖醛酸苷键加水分解的酶,它可以把葡萄糖醛酸轭合物或硫酸轭合物的糖苷键切断,释放出游离态。目前,β-受体激动剂类药物残留的检测方法包括高效液相色谱法(HPLC)、气相色谱-质谱法(GC-MS)、液相色谱-质谱/质谱法(LC-MS/MS)、毛细管电泳电气化学检测法。下面介绍使用新型重组酶 IMCSzyme® β-葡萄糖醛酸酶与 MicroSep® DPX 枪头式分散固相
体 对于包涵体的纯化,包涵体的前处理是很重要的。 包涵体中主要含有重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂,如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇(DTT)、β-巯基乙醇等反复多次进行,因去垢剂洗涤能力随溶液离子强度升高而加强,在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上,而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去
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