- ￥1500 - 3280
- Proteintech
- 83795-7-RR
- 2025年10月09日
- WB, IHC, ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
IL-6
- 抗体英文名：
IL-6 Recombinant monoclonal antibody
- 抗体名：
IL-6 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | PMA, LPS and Brefeldin A treated RAW 264.7 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83795-7-RR targets IL-6 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0973 Product name: Recombinant Mouse IL-6 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 25-211 aa of NM_031168 Sequence: FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 6 |
| 别名 | Il6, Il 6, interleukin 6, interleukin-6 |
| 计算分子量 | 24 kDa |
| GenBank蛋白编号 | NM_031168 |
| 基因名称 | Il6 |
| Gene ID (NCBI) | 16193 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P08505 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
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文献和实验ListeningRain 本人欲买猪重组细胞因子白介素6（IL-6)和肿瘤细胞坏死因子（TNF-a)，在网上搜集以及和试剂公司联系了很久，发现只有以色列的Prospec的规格比较适合！RD公司的只有大包装25ug(3000元左右）（太贵，而且我用的量比较少），Prospec有2ug和5ug，价格在600元左右，容易接受。 我是要在体外细胞实验中加入这两种细胞因子，作为干预措施，观察细胞对它们的反应性！ 不知，有用过这家公司细胞因子的丁友
图上查出相应 IL-6 含量。 试剂盒性能 1. 灵敏度 ： 最小的 IL-6 检测浓度小于 16pg/ml 。 2. 特异性： 可同时检测重组或天然的大鼠 IL-6 。 不与 大鼠其它细胞因子有交叉反应。 3. 重复性：板内、板见变异系数均小于10 ％。 注意事项 1. 以上标准孔及待测样品均建议做复孔，每次测定应同时做标准曲线。
相应 IL-6 含量。 试剂盒性能 1. 灵敏度 ： 最小的 IL-6 检测浓度小于 15pg/ml 。 2. 特异性： 可同时检测重组或天然的狗 IL-6 。 不与 狗其它细胞因子有交叉反应。 3. 重复性：板内、板见变异系数均小于10 ％。 注意事项 1. 以上标准孔及待测样品均建议做复孔，每次测定应同时做标准曲线。 2. 洗涤
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