- ￥1500 - 3280
- Proteintech
- 82828-12-RR
- 2025年10月09日
- WB, Dot Blot, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, Dot Blot, ELISA
- 靶点：
Di-Methyl-Histone H3 (Lys56)
- 抗体英文名：
Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody
- 抗体名：
Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive Dot Blot detected in | peptide |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82828-12-RR targets Di-Methyl-Histone H3 (Lys56) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Dot Blot, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3 |
| 观测分子量 | 15-18 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).
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文献和实验Reagents 100-bp DNA size ladder (Promega) Agarose Antisera (affinity-purified) These should be raised against histone peptides with mono-, di-, or trimethylation or acetylation at a specific lysine/arginine residue
查到最佳抗体稀释比例，可在芯片研究工作开始之前，通过 Western 印迹实验来确定。 ( 12 ) 为了防止芯片在与抗体孵育的过程中干燥，我们把芯片放在用 TBST 润湿的 Whatman 滤纸上。 ( 13 ) 由于二抗被突光标记，因此，这一步骤以及其后的每个步骤实验均需在黑暗中进行。 ( 14 ) 荧光标记的二抗 4°C 下保存，或加入甘油（终浓度为 50% ) 保存在 一20°C。 ( 15 ) 我们采用另一种在 FAST 片基上筛选单克隆抗体的方法：FAST 片基室温
9/t0+7hkd4kpA+zZC9eknYPv27N7jQG93KdnpnHmbGM33vf9wNAZ6ffmgnYULc+XLRgblx+NlaeZ+iwL722ZOygC4FgukX7oR09r12bnj56aKiWHc0919bdAPDlLVbpo1Zu6ZDbeBSvLl++vMpzvEA+ku0GONqWvNYudLX0gXQLwA3uuLvcdtttdSaRLP6DP/gX4RnnG3SnXMpTPeRBdtFDgj4KMLc+e/BgZy1Wron9T3nlezj811HgMEj
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