- ￥1500 - 3280
- Proteintech
- 84599-5-RR
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
CD46
- 抗体英文名：
CD46 Recombinant monoclonal antibody
- 抗体名：
CD46 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84599-5-RR targets CD46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1770 Product name: Recombinant Human CD46 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 35-328 aa of NM_172351.3 Sequence: CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD46 molecule, complement regulatory protein |
| 别名 | 241764F8, MCP, Membrane cofactor protein, MIC10, TLX |
| 计算分子量 | 42kDa |
| 观测分子量 | 50-65 kDa |
| GenBank蛋白编号 | NM_172351.3 |
| 基因名称 | CD46 |
| Gene ID (NCBI) | 4179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P15529-11 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).
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文献和实验CD46 分子 CD46 常用单克隆抗体或代号： HULYM5,J48 主要表达细胞： 广泛,Leu,Pt [NL] 分子质量(kDa)和结构： gp56- 66,膜辅助因子蛋白(MCP) 功 能： 调节补体活化,裂解C3b ,C4b膜辅助因子蛋白 CD46 Aka membrane cofactor protein (MCP) Cofactor for Factor
ml）lml，再从鸡的翼下静脉取血0.5--1ml，取出后放入刻度离心管中，再加入2--3ml Alsver液，使总量为4--5ml，混匀并封口，置于4℃冰箱内可供一周内使用，实验时取贮备的鸡血细胞lml，加入4ml85％生理盐水，混匀后以1200rr/min离心5min，去上清液后，最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次，最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞，可先用0.9％
CD抗原及其相应的单克隆抗体在基础和临床免疫学研究中已得到广泛的应用。在基础免疫学研究中，CD主要应用于： （1）CD抗原的基因克隆，新CD抗原及新配体的发现； （2）CD抗原结构与功能关系； （3）细胞激活途径和膜信号的传导； （4）细胞分化过程中的调控； （5）细胞亚群的功能。 在临床
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