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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 保存条件:
Store at 2-8°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
rat
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD11b/c
- 抗体英文名:
Anti-Rat CD11b/c (OX42)
- 抗体名:
Anti-Rat CD11b/c (OX42)
- 规格:
100ug/500ug
| 规格: | 100ug | 产品价格: | ¥1070.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥3340.0 |
经过测试的应用
| Positive FC detected in | LOU rat splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65229-1-Ig targets CD11b/c in FC applications and shows reactivity with rat samples.
| 经测试应用 | FC |
| 经测试反应性 | rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha M |
| 别名 | CD11b, integrin alpha M, Itgam, OX42 |
| 计算分子量 | 127 kDa |
| GenBank蛋白编号 | NM_012711 |
| 基因名称 | CD11b |
| Gene ID (NCBI) | 25021 |
| RRID | AB_3084908 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q9JI30 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).
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Platelet-associated Ig (PAIg) Elution
=2.5) to a final concentration 2-10 x 10^8 Platelets/mL Incubate at RT for 15 min. Centrifuge at 14000 rpm, 5min , RT or 10ѓC Neutralize with 1mol/L Tris-base (pH=9.0) Dialyze ON, 4ѓC, against PBS
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