FcZero-rAb™ PerCP-Cyanine5.5 R
abbit IgG Isotype Control Recombinant Antibody CPY5-FcA98136- ￥1680 - 3360
- Proteintech
- CPY5-FcA98136
- 2025年10月09日
- FC
- Rabbit
- n/a
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
PerCP-Cyanine5.5 Fluorescent Dye
- 适应物种：
n/a
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
IgG Isotype Control
- 抗体英文名：
FcZero-rAb™ PerCP-Cyanine5.5 Rabbit IgG Isotype Control Recombinant Antibody
- 抗体名：
FcZero-rAb™ PerCP-Cyanine5.5 Rabbit IgG Isotype Control Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥1680.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥3360.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-FcA98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IgG Isotype Control |
| 别名 | 240953C9, Rabbit IgG, Rabbit IgG Control, Rabbit IgG Isotype, Rabbit IgG Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
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