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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
Human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD38
- 抗体英文名:
CoraLite® Plus 750 Anti-Human CD38 (HIT2)
- 抗体名:
CoraLite® Plus 750 Anti-Human CD38 (HIT2)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥920.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2310.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65111 targets CD38 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD38 molecule |
| 别名 | ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1 |
| 计算分子量 | 300 aa, 34 kDa |
| GenBank蛋白编号 | BC007964 |
| 基因名称 | CD38 |
| Gene ID (NCBI) | 952 |
| ENSEMBL Gene ID | ENSG00000004468 |
| RRID | AB_3673696 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P28907 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).
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文献和实验在检测一些特殊的核内指标时,一般都会有推荐的固定破膜方案。比如在 Ki- 67 染色一般会推荐用 70% 的冰乙醇作为固定剂(含打孔作用),如前文提到的脱水剂类固定剂对蛋白空构象的影响大,在单染 Ki- 67 的时候,乙醇的固定方式是完全没有问题的,但是在和其它指标共染时,就需要考虑乙醇固定的方式和其它抗体兼容性的问题,比如 CD38 (Clone: HIT2) 的抗体是明确不能和乙醇固定的方式兼容,而 CD34 (Clone: 581) 很大程度上可以兼容这种固定方式。醇类和其它抗体(Clone
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