- ￥1350 - 3000
- Proteintech
- 19760-1-AP
- 2025年10月09日
- WB, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
GPR42P
- 抗体英文名：
GPR42P Polyclonal antibody
- 抗体名：
GPR42P Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HuH-7 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19760-1-AP targets GPR42P in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 42 pseudogene |
| 别名 | FFAR1L, GPR41L, GPR42 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | NM_005305 |
| 基因名称 | GPR42P |
| Gene ID (NCBI) | 2866 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15529 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR42, also named as GPR42P, FFAR1L and GPR41L, belongs to the G-protein coupled receptor 1 family. GPR42 appears to have arisen by tandem duplication of FFAR3 and have lost the ability to activate the G-protein family in response to carboxylate ligands primarily due to an amino acid change at position 174. It is an orphan receptor.
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文献和实验通路是通过磷酸化激活下游的，磷酸化的就是活化形式啦，比如c-jun吧，有钱的话还是要要买c-jun和p-c-jun的,紧张的话但用p-c-jun也能说明问题，里面的各型无非就是多抗，兔或鼠单抗，兔单抗比较好，选择的话还要看你做什么用途，个人浅见 chuckdouble superman211 wrote: 拟通过WB进行AP-1的相关研究，在选择一抗时遇到如下困惑，望了解的战友予以解答。关于AP-1的出c-jun的一抗有多种，其中主要
Regulation and Control of AP-1 Binding Activity in Embryotoxicity
is generally detected with autoradiography of the 32 P-labeled DNA. The underlying principle is that nucleic acid with protein bound to it will migrate more slowly through a gel matrix than the free nucleic acid. In this chapter, a representative protocol
-FGF-heparin sepharose complex was transferred in 60 µl 10 mM Tris pH 7.5, to a well of a microtiter plate containing 60 µl of 2« AP assay buffer (2 M diethanolamine, 1 mM MgCl2, 20 mM homoarginine, 12 mM p-nitrophenyl phosphate pH 9.5). The bound
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