- ￥5800
- Proteintech
- 83784-6-PBS
- 2025年10月09日
- WB, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Islet 1 protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Islet 1
- 抗体英文名：
Islet 1 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Islet 1 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
83784-6-PBS targets Islet 1 as part of a matched antibody pair:
MP00771-4: 83784-7-PBS capture and 83784-6-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33541 Product name: Recombinant human Islet 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC031213 Sequence: MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA* |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ISL LIM homeobox 1 |
| 别名 | ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39-45 kDa |
| GenBank蛋白编号 | BC031213 |
| 基因名称 | Islet 1 |
| Gene ID (NCBI) | 3670 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P61371 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.
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文献和实验50μL。 封闭液：用PBS将试剂盒中的封闭存储液（Blocking stock solution R）稀释10倍，每孔200μL。 抗体稀释液：注意，只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液（Dilution buffer R）稀释10倍 检测抗体（Biotinylated detector antibody，Secondary antibody）：照U-Cytech说明书，加入双蒸水溶解。使用时，用抗体稀释液稀释100倍，每孔100μL。 酶联亲和
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