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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CDH17 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF-P, ELISA
- 靶点:
Cadherin-17
- 抗体英文名:
Cadherin-17 Recombinant monoclonal antibody
- 抗体名:
Cadherin-17 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | mouse colon tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human stomach cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83793-5-RR targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19488 Product name: Recombinant human CDH17 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 89-406 aa of BC113464 Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cadherin 17, LI cadherin (liver-intestine) |
| 别名 | CDH17, HPT1, HPT 1, CDH16, Cadherin 17 |
| 计算分子量 | 832 aa, 92 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC113464 |
| 基因名称 | Cadherin-17 |
| Gene ID (NCBI) | 1015 |
| RRID | AB_3671384 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q12864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.
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文献和实验百种蛋白质可以诱发信号,血清反应因子的几个靶基因也是已知的 SRF 本身的上游诱导剂。其中,成纤维细胞生长因子 8(Fgf8)和 Fgf17 呈现了最强的剂量依赖性反应。他们随后将 Fgf17 作为深入研究的重点,因为它是一种大脑富集蛋白,其在人和小鼠神经元中的水平会随着年龄的增长而下降。 图片来源:Nature 初步的体外实验结果表明,Fgf17 可显著诱导 OPCs 的增殖。 为了测定它们的体内活性,他们将重组 Fgf8 和 Fgf17 注入老龄小鼠,类似于脑脊液的给药方式。体内
在体外将两个或多个来源相同或不相同的 DNA 片段连接成新的重组 DNA 分子,再转到特定宿主细胞中进行自主复制并表达。这是分子生物学中的基本技术。DNA 重组技术的基本程序包括:(1)获得外源 DNA:外源 DNA 是进行 DNA 重组的目的 DNA 片段,一般采用 DNA 聚合酶链式反应 (PCR) 或逆转录-DNA 聚合酶链式反应 (RT-PCR)、基因组文库或 cDNA 文库筛选等方法获得。(2)载体的构建或选择:分子生物学中用的载体是指能在特定宿主细胞中自主复制的 DNA 分子
CD17 分子 CDw17 常用单克隆抗体或代号: GO35 lactosylCeramine 主要表达细胞: G,M,Pt [M] 分子质量(kDa)和结构: 乳糖基酰鞘氨醇 功 能: 可能参与吞噬和信号转导 CD17 Aka Lactosylceramide Binds to bacteria
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