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- Proteintech
- CL750-83137-4
- 2025年10月09日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RNF31 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
Human
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
RNF31 / HOIP
- 抗体英文名：
CoraLite® Plus 750-conjugated RNF31 / HOIP Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 750-conjugated RNF31 / HOIP Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83137-4 targets RNF31 / HOIP in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag34505 Product name: Recombinant human RNF31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 459-603 aa of BC017376 Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 31 |
| 别名 | FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA |
| 计算分子量 | 1072 aa, 120 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC017376 |
| 基因名称 | RNF31 |
| Gene ID (NCBI) | 55072 |
| RRID | AB_3673771 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96EP0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
2 O, add 250 ml 1 M Tris ・ Cl [pH 7.4] stock solution, dissolve 43.83 g NaCl, dH2 O ad 500 ml) 2 x SSC (50 ml of 20 x SSC stock solution , dH2 O ad 500 ml) Procedure: Turn on the vacuum oven. Cut 4 pieces
包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 1.2.5 DNA片段分析 SGC-7901感染Ad-p27mt和Ad-LacZ 48 h后离心去上清, 在剩余细胞
，用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2，37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG，FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法，采用抗体通用的兼并性引物，从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因（VL和VH）后，利用编码短肽（G4S）3的连接链DNA片段，经overlap PCR，将VH和VL基因连接构成ScFv基因（约750bp
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