- ￥5800
- Proteintech
- 68023-1-PBS
- 2025年10月09日
- WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
- 靶点：
Phospho-EIF2S1 (Ser51)
- 抗体英文名：
Phospho-EIF2S1 (Ser51) Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Phospho-EIF2S1 (Ser51) Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
68023-1-PBS targets Phospho-EIF2S1 (Ser51) as part of a matched antibody pair:
MP50181-1: 68479-1-PBS capture and 68023-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
| 别名 | EIF2S1 (Ser51), p EIF2S1, p EIF2S1 (Ser51), p EIF2S1 Ser51, p-EIF2S1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | NM_004094 |
| 基因名称 | EIF2S1 |
| Gene ID (NCBI) | 1965 |
| RRID | AB_2918767 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05198 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
； 2、一抗用多克隆抗体易出现非特异性着色，建议用单克隆抗体看看； 3、内源性过氧化物酶和生物素在肝脏、肾脏等组织含量很高（含血细胞多的组织），需要通过延长灭活时间和增加灭活剂浓度来降低背景染色； 4、非特异性组分与抗体结合，这需要通过延长二抗来源的动物免疫血清封闭时间和适当增加浓度来加强封闭效果； 5、缩短 DAB 孵育时间或降低 DAB 浓度/过氧化氢浓度等； 6、适当增加 PBS 冲洗次数和浸洗时间，在一抗、二抗或 SP 孵育之后的浸洗尤为重要； 7、防止标本染色过程中
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