- ￥2800
- Proteintech
- CL488-67835
- 2025年10月09日
- IF/ICC, IF-P, FC (Intra)
- Mouse
- human, mouse, rat, pig, rabbit
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human ELAVL4 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig, rabbit
- 宿主：
Mouse
- 应用范围：
IF/ICC, IF-P, FC (Intra)
- 靶点：
ELAVL4
- 抗体英文名：
CoraLite® Plus 488-conjugated ELAVL4 Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated ELAVL4 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67835 targets ELAVL4 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag3678 Product name: Recombinant human ELAVL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC036071 Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVGQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAITSLNGYRLGDRVLQVSFKTNKAHKS |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| 别名 | HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4 |
| 计算分子量 | 380 aa, 42 kDa |
| 观测分子量 | 32-40 kDa |
| GenBank蛋白编号 | BC036071 |
| 基因名称 | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2923797 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26378 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
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